M.Sc. (Food Chemistry and Biochemistry), University Putra Malaysia
B.Sc. (Food Science and Technology), University Putra Malaysia
Hometown: Georgetown, Penang, Malaysia
Project: Development of Sensitive Immunoassays for the Detection of 2-Methylisoborneol and Monensin in Environmental and Food Samples
- To develop immunoassays with a high sensitivity and specificity for rapid detection of 2-methylisoborneol (MIB) in freshwater at 5-10 pg/mL (ppt) level.
- To develop immunoassays with a high sensitivity and specificity for detection of monensin in food samples.
- To validate the immunoassays developed for MIB and monensin using environmental and food samples.
2-methylisoborneol (MIB) is a secondary metabolic product of some species of blue-green algae and actinomycete, filamentous bacteria and fungi, and a major cause of off-flavour in potable water and aquaculture industries. Monensin is a polyether ionopore antibiotic, used in veterinary medicine for coccidiosis treatment and prevention in poultry and cattle, and a growth promoter in livestock. Both are hydrophobic haptens with molecular weights of 168 (MIB) and 670 Da (monensin). (-)Camphor conjugated to BSA, and MIB conjugated to thyroglobulin (TBG) were made for MIB immunogens and plate coatings. Monensin was conjugated to BSA and OVA for immunogen and plate coatings, respectively. Fourteen monoclonal antibody clones were isolated through hybridoma technology for MIB. Limit of detection (LOD) for MIB was produced through inhibition ELISA and was 0.01 ng/mL for mAb 4F11 clone and 5 ng/mL for polyclonal antibody obtained from rabbit serum. The LOD for monensin, as determinded using a competitive-indirect (CI)-ELISA, was 0.1 ng/mL using rabbit serum through. These assays will be further optimized followed by determination of accuracy, precision, specificity, while assay robustness will be determined by measuring the effects of various matrices, solvents, and pHs. Food and environmental samples will be analysed using the ELISA and compared with liquid and gas chromatography/MS results for validation of the assay.